Lectin-Based Method for Deciphering Human Milk IgG Sialylation

Lectin-Based Method for Deciphering Human Milk IgG Sialylation

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In light of the immunoprotective function of human milk and the incontestable impact of IgG glycosylation Desk w/Bench on its immune functions, characterization of the sialylation profile of human milk IgG is needed.Lectins as a molecular probe were applied in lectin-IgG-ELISA to analyze the sialylation and galactosylation pattern of skim milk IgG of mothers who delivered at term and prematurely.Well-defined biotinylated lectins were used: Maackia amurensis II (MAA II), Sambucus nigra (SNA), Ricinus communis I (RCA I), and Griffonia simplicifolia II (GSL II) specific to α2,3-Neu5Ac, α2,6-Neu5Ac, Gal(β1,4)GlcNAc, and agalactosylated glycans, respectively.The sialylation pattern of milk IgG differs qualitatively and quantitatively from maternal plasma IgG and is related to lactation stage and perinatal risk factors.

Expression of MAA-, SNA-, and GSL-reactive glycotopes on term milk IgG showed a positive correlation with milk maturation from days 1 Brain Health to 55.Preterm birth was associated with an increase of MAA-reactive and a decrease of RCA-reactive IgG glycotopes.Moreover, higher SNA- and GSL-reactive and lower RCA-reactive glycoform levels of milk IgG were associated with infection of lactating mothers.Application of a specific and simple method, lectin-IgG-ELISA, reveals the sialylation pattern of milk IgG over milk maturation.

However, further investigations are needed in this area.